User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15

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Results last samples produced valid results when compared with positive controls Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using. Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control 1ul F primer 1ul R primer 2.0ul dNTPs 0.5u Taq 1ul DNA 2.5ul 10x buffer 1.5ul MgCl2 16ul H2O 1:1SWWBD 2:2SWWBD 3:R5#9 4:R5#51 5:R5#52 6:R5#100 7 :Pink Gold male female neg Results: no bands. Try lower annealing temperature - say 55°C