User:Stuart McKellar/Notebook/Bird Sex Testing/2012/11/13
|Project name||Main project page|
Previous entry Next entry
If the PCR failed, I expect the DNA not to migrate. I am loading 5ul DNA sample with 1ul x 6x DNA running buffer (glycerol + bromophenol blue).
Samples were loaded as follows:
Gel ran at 100V (intially started at 300V for a few seconds and then pansied out) and 60mA. Immediate illumination could be seen in the well with the ladder. Gel is running and I can see the ladder but nothing else. Fuck. Looks like I am going back to PCR. After 300bp had reached centre, still no light from samples. Maybe run a single lorikeet sample through PCR and then check on a gel.