User:Stuart McKellar/Notebook/Bird Sex Testing/2012/10/19

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Electrophoresis Test Objective: Try and visualise DNA on an agar gel. Method: Poured a 2% Gel pre-stained with GelRed 1:10,000. This was from a while ago so I may need to post stain it. Heated in microwave for a couple of minutes and then let cool. Poured gel, added 3 x 3.3ul of 300-10KB ladder. Added 1 x 5ul ladder in 4th lane. Ran gel at 60ma and 100V (volts as recommended). Results: DNA had visibly moved in first few minutes. Gel box underneath was on but no visualisation yet. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though. No visible results. This could be for the following reasons: The ladder is degraded The GelRed is not emitting light at a visible frequency There is not enough contrast The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue And that is all the reasons that I can think of.