User:Stuart McKellar/Notebook/Bird Sex Testing/2012/09/11
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Notes from last time
DNA Extraction for today
Doing all the loris and tawny's and the two other samples picked up today.
And the brown doves
I am mixing up a 12x mix of solution 1a and 1b for the EDNA kit. 64ul+16ul is 80ul per tube. Times 12 is 768 of 1a and 192 of 1b.
Tawnys are fine to DNA sex test (check your bookmarks for the link). Also, the diamond doves may have a problem, as only the males will show up. Maybe in future use a house keeping gene or design some primers around the sequence and get it sanger sequenced.
DNA extraction seems to have worked fine, added the solution 2 and put them in the fridge.