User:Steven J. Koch/Notebook/Kochlab/2009/06/12/Freezer, fridge box inventory

Steve Koch 17:33, 12 June 2009 (EDT): We need to establish some kind of easy to use inventory system soon. Anthony is going to ask Bill about the barcode system we talked about a couple years ago. That could possibly be awesome. Until we have a system, though, I am copying over some information I had on the private wiki about my freezer boxes:

SJK Fridge box (+4C)

• Magnetic microspheres stocks
  • M-270 streptavidin 653.05, LOT G17100 EXP2008-01
  • M-280 Streptavidin 112.05, LOT F92640 EXP 2007-09
  • My One Carboxylic 650.11, LOT G64810 EXP 2007-05
  • M-270 Carbox 143.05 LOT G52510 EXP 2007-09
  • My One Strept 650.01, LOT G29100 EXP 2006-12
• Polysciences NIST traceable megabeads Lot #559157, cat. no 64170, 30.25 +/- 0.065 micron
• Some really old BGB
• Some dragon green dilution
• Some fork constructs and working solutions (very old) for tethering
• 1 mg / ml polylysine aliquots

Steve -20C #2

• 10/31/05: 1192 D/B; 1211 D/B; 1411 D/B; 4430 D/B
• 7/7/04: pRL574 ~150ng/ml, tubes 2, 3, 4
• pUC19 DNA, 100 pg/ul Invitrogen
• Epicentre rNTPs, Cat. No R344NT
• Epicentre individual rNTPs, all four nts
• Oligos:
  • 5-BioFork1
  • 3-DigFork1
  • pRl574-R2044; F834-dig; F834; R2008; R2008-bio; R2044-bio
  • 10 uM dilutions
• 10XPCR buffer
• 1:100 pRL574 dilution
• 2.5mm dNTPs
• 500X amp

Steve -80C #1 (now in -20C, though)

• pRL574 glycerol stocks
• dNTPs
• Oligos
  • pRL574-R5263-bio; R5263; F853-dig; F853
• Some kind of kinesin glycerol stock (strains I think)
• Drosophila kinesin from 2/10/04
• unidentified tubes labeled with alpha or gamma

Antidig box

• 20 ul aliquots of antidig