User:Stefan J. Tekel/Notebook/Pc-TF Engineering/2015/10/16

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Pc-TF Expression

25 mL LB/kan with OD600= 0.1 of overnight culture of pET28-PcTF

Induce with 1 mM (final) of IPTG when OD600 = 0.8

Grow 2 hours at 37 C in shakeubator

Pelleted cells and can visually see red fluorescence

File:10.16.15 PcTF test expression.TIFF