User:Ssutton/Phospholocator/Vectors
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Samantha Box 1: Phospholocator vectors
| Box | Name | Construct Description | Plasmid type | Auxotrophy (Yeast) | Antibiotic resistance | Promoter | Terminator | To integrate, cut with: |
| Samantha Box 1: Phospholocator vectors | SSY416GHI | Full-length Cdc28-Phosphoplocator | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY416GHC | Full-length Cdc28-Phosphoplocator | Centromeric | His3 | Amp | Gal1 | Cyc1 | - |
| Samantha Box 1: Phospholocator vectors | SSY429GHI | Full-length S15A-Cdc28-Phospholocator (first serine mutated to alanine) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY429GHC | Full-length S15A-Cdc28-Phospholocator (first serine mutated to alanine) | Centromeric | His3 | Amp | Gal1 | Cyc1 | |
| Samantha Box 1: Phospholocator vectors | SSY430GHI | Full-length S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY430GHC | Full-length S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) | Centromeric | His3 | Amp | Gal1 | Cyc1 | |
| Samantha Box 1: Phospholocator vectors | SSY431GHI | Full-length S33A-Cdc28-Phospholocator (second serine mutated to alanine) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY431GHC | Full-length S33A-Cdc28-Phospholocator (second serine mutated to alanine) | Centromeric | His3 | Amp | Gal1 | Cyc1 | |
| Samantha Box 1: Phospholocator vectors | SSY432GHI | Full-length S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY432GHC | Full-length S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) | Centromeric | His3 | Amp | Gal1 | Cyc1 | |
| Samantha Box 1: Phospholocator vectors | SSY416tGHI | Abridged Cdc28-Phosphoplocator | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY429tGHI | Abridged S15A-Cdc28-Phospholocator (first serine mutated to alanine) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY430tGHI | Abridged S15D-Cdc28-Phospholocator (first serine mutated to aspartic acid) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY431tGHI | Abridged S33A-Cdc28-Phospholocator (second serine mutated to alanine) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY432tGHI | Abridged S33D-Cdc28-Phospholocator (second serine mutated to aspartic acid) | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY445GHI | Full-length Fus3-Phospholocator | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
| Samantha Box 1: Phospholocator vectors | SSY445GHC | Full-length Fus3-Phospholocator | Centromeric | His3 | Amp | Gal1 | Cyc1 | |
| Samantha Box 1: Phospholocator vectors | SSY445tGHI | Abridged Fus3-Phospholocator | Integrating | His3 | Amp | Gal1 | Cyc1 | NheI |
