User:Sim Huay Ping/Notebook/CBE/08/150/2008/09/04

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Use of the Spectrometer to determine cell count

1. Fill cuvette with bacteria inoculum and buffer

2. Put the cuvette into the spectrometer at 540 nm for reading

3. Obtain an OD value of 0.05 (cell count of 10 to the power of 7)

4. Always obtain this OD reading for every experiment for consistency