User:Sean P Corum/Notebook/PHIX174 Cell Free/2012/07/09

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Characterization B: Expression of PHIX174 promoters fused to UTR1-deGFP.

  • I transformed pBEST-PA-UTR1-deGFP-T500 ligation product into JM109. Plates showed many colonies. -linker negative control showed fewer colonies, as expected. Six colonies were picked from the plate for miniculture.

Characterization C: Expression of PHIX174 promoters fused to UTRX-deGFP.

  • I transformed pBEST-PA-UTRA-deGFP-T500 ligation product into JM109. Plates showed many colonies. -linker negative control showed fewer colonies, as expected. Six colonies were picked from the plate for miniculture.

Hypothesis 2: Gene L is necessary for phage propagation.

  • Whole plasmid PCR works with the control plasmid and primers included in QuickchangeII kit.
  • 5 nM ΦX174 template is functional in regular PCR.
  • Therefore, the problem is with my primers.