User:Sayed Massie Husaini/Notebook/september 5 2017/2017/10/17
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Reducing agent stock and AuNP solutionsPrepare reducing agent stock solutions 5 - 10 mL of 100 mM each fructose, galactose, sucrose, Na3Ct, H2Asc For chitosan (168 g/mol), prepare 10 mL 50 mM. Add 4.75 mL HCl per g of chitosan or 0.265 mL HAc per g of chitosan For cellulose nanocrystals, use a 4% solution 1 mg NaBH4 in 20 mL ice cold H2O. Not capped AuNP for 100 mM stock solutions 0.1 mL 10 mM HAuCl4 + 0.1 mL 100 mM reducing agent + 3.8 mL H2O For cellulose, add 0.1 mL 10 mM HAuCl4 to 3.9 mL cellulose 0.2 mL 10 mM HAuCl4 + 3.8 mL NaBH4 Place test tubes in water bath (600 mL beaker) at 80 C until color changes (~30 min) Measured UV-VIS of your solutions
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