User:Sarah Haack/Notebook/Capstone Research/2012/02/16

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February 16, 2012

  • Today's research examined the capabilities of a new, smaller UV spectrometer instrument. The purpose of the research was to determine whether the two instruments would be equivalent in measuring and recording absorbance of light by the protoporphyrin solutions. This spectrometer measured light intensity, and not absorbance, so the log(I0/I) of the values had to be taken, where I0= the intensity of the cuvette of pure DMF and I= the intensity of the solution sample.

The previously tested samples of diluted nickel and DMF, and nickel and DMF with added ascorbic acid, were run through the new instrument. The spectrometer was calibrated to an integrated time of 8 milliseconds and a scanning average of 25 in order to produce more desirable values. The results were plotted in graphs next to one another, and revealed a great difference in the instruments.