User:Sarah Burkhard/Notebook/471 Nano Notebook/2017/04/11

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Objectives

  • make buffer solutions for pH 10 - 13 to keep ionic strength constant
  • make solutions pH 4 - 13 for Arg, Gln, His, capped Arg
  • sequence of adding: Gold, AA, buffer

Observations

  • Arg solution turned yellow for a split second upon combing gold and AA
  • Gln lost yellow color
  • His became slightly cloudy
  • upon adding buffer, Gln solution regained yellow color for a split second

Notes

  • Cys - Au polymer shape is pH dependent. At high pH, Au - S - Au bridge elongates, reduces Au - Au interaction and no peak is visible in spectroscopy