User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/14

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Ocean Optics Round 2 pH 5 Objective Draw conclusions on reaction speed of nanoparticle formation by measuring absorbance of light, every 2 minutes over a period of 3 hours. Sample Solution Stocks 4.57 mM Au 53.3 mM BSA 0.001 mM HCl DI Water Volumes Added to Generate Sample 175.89 uL BSA 164.11 uL Au 30 uL HCl 2629.99 uL HOH Protocol The Protocol can be found at the AU Design Lab Data Analysis The increase in absorbance is proportionally and time-connected to the formation of AU nano particles. The shift in wavelength absorbance indicates the formation of free AU nano particles (linked to the unfolded Protein)