User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/18

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Fluorescence

Purpose

Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 7

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.95mM
      1. (0.25mM)(3.0mL)=(XmL)(3.95mM)
      2. XmL= .190mL= 190uL Au
    2. Myoglobin 133uM
      1. (12.5uM)(3.0mL)=(XmL)(133uM)
      2. X=0.282mL= 282uL Myoglobin
    3. Water
      1. Volume of water= 3000uL- 190uL-282uL= 2528uL
  2. Absorbance measurements were taken and saved every 3 minutes for 3 hours
  3. The instrument was set at 80 degrees Celsius
  4. The scan range parameters were start at 310nm and ending at 540nm
  5. The emission and exit slit were set at 10nm

Data

Scans AuNP Myoglobin pH7

Scans of myoglobin AuNP pH7.png

Wavelength of Myoglobin AuNp's at pH7

Wavelength myoglobin AuNP pH7.png

Maximum Intensity of Myoglobin AuNp's at pH7

Max intensity Myo AuNP pH7.png

Integrated Intensity of Myoglobins AuNp's at pH7

Integrated intensity AuNP myo pH7.png

Solution for UV-Vis Preparation

Stock Concentrations:

  1. Myoglobin 133uM
  1. Silver 25.8mM
  1. Fructose 33.0mM


0.125mM Fructose
pH μL of 1 mM HNO3stock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AgNO33stock μL of Fructosestock μL of Myoglobinstock μL of Water
4 500 0 0 48 19 470 3962
5 50 0 0 48 19 470 4412
6 5 0 0 48 19 470 4457
7 0 0 0 48 19 470 4462
8 0 5 0 48 19 470 4457
9 0 50 0 48 19 470 4412
10 0 500 0 48 19 470 3962
11 0 0 5 48 19 470 4457
12 0 0 50 48 19 470 4412


0.25 mM Fructose
pH μL of 1 mM HNO3stock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AgNO33stock μL of Fructosestock μL of Myoglobinstock μL of Water
4 500 0 0 48 39 470 3943
5 50 0 0 48 39 470 4393
6 5 0 0 48 39 470 4438
7 0 0 0 48 39 470 4443
8 0 5 0 48 39 470 4438
9 0 50 0 48 39 470 4393
10 0 500 0 48 39 470 3943
11 0 0 5 48 39 470 4438
12 0 0 50 48 39 470 4393