User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/12

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Ocean Optics

Purpose

To use ocean optics to make conclusions about the rate of myoglobin templated Au nanoparticle formation in pH7.

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.18mM
      1. (0.25mM)(3.0mL)=(XmL)(3.18mM)
      2. XmL= .236mL= 236uL Au
    2. Myoglobin 133uM
      1. (12.5uM)(3.0mL)=(XmL)(133uM)
      2. X=0.282mL= 282uL Myoglobin
    3. Water
      1. Volume of water= 3000uL- 236uL-282uL= 2482uL
    4. Scans were taken at 80oC every 2 minutes for 3 hours

Data

Absorbance of Myoglobin Au NP's at pH7

Myoglobin pH7 AuNP.png

Wavelength of Myoglobin Au NP's at pH5

Myoglobin pH7 AuNP wavelength.png