User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/13

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Ocean Optics

Purpose

To use ocean optics to make conclusions about the rate of nanoparticle formation in pH5.

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 0.1mM HCl 3mL total
    1. Au 4.57mM
      1. (0.25mM)(3.0mL)=(XmL)(4.57mM)
      2. XmL= .164mL= 164uL Au
    2. Myoglobin 137uM
      1. (12.5uM)(3.0mL)=(XmL)(137uM)
      2. X=0.274mL= 274uL Myoglobin
    3. HCl 0.1mM
      1. (3.0mL)(0.01mM)=(1.0mM)(XmL)
      2. XmL=0.03mL= 30uL HCl
    4. Water
      1. Volume of water= 3000uL- 164uL-274uL-30uL= 2532uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours

Scans

Absorbance at pH5

px600

Wavelength at pH5

px600