User:Sanuja Mohanaraj/Notebook/Experimental Biochemistry 1 Fall 2017/2017/10/17

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Reducing Agent Stock Solutions Preparation

10mL of 100mM of fructose, galactose, sucrose, Na3Ct, H2Asc, and cellulose nanocrystal were prepared to the calculations below. As for chitosan, a 10mL (50mM) solution was prepared similarly, followed by the addition of 0.017mL HCl. Next, 1mg of NaBH4 was placed in 20mL ice cold deionized water, without capping.

Gold nanoparticle solutions were then prepared using each of the above prepared reducing agent stock solutions (in separate test tubes) according to the following:
0.1mL 10mM HAuCl4 + 0.1mL 100mM reducing agent + 3.8mL H2
As for cellulose, 0.1mL of the 10mM gold stock solution was added to 3.9mL of the prepared cellulose solution. Next, 0.2mL of the gold stock solution was added to 3.8mL of the prepared NaBH4 reducing agent stock solution.

Each of the prepared seven test tubes containing the different gold nanoparticle solutions, were then placed in a water bath using a 600mL beaker at 80°C for approximately thirty minutes.

Calculations

Fructose and Galactose Reducing Agent Stock Solutions
100mM = moles / 10mL
moles = 0.001 moles
(0.001 moles)(180.156g/mol) = 0.180156 grams

Chitosan Reducing Agent Stock Solution
50mM = moles / 10mL
moles = 0.0005 moles
(0.0005 moles)(168g/mol) = 0.084 grams

Sucrose Reducing Agent Stock Solution
100mM = moles / 10mL
moles = 0.001 moles
(0.001 moles)(342.296g/mol) = 0.034296 grams

Cellulose Nanocrystals Reducing Agent Stock Solution
100mM = moles / 10mL
moles = 0.001 moles
(0.001 moles)(162g/mol) = 0.162 grams

Na3Ct Reducing Agent Stock Solution
100mM = moles / 10mL
moles = 0.001 moles
(0.001 moles)(294.1g/mol) = 0.2941 grams

H2Asc Reducing Agent Stock Solution
100mM = moles / 10mL
moles = 0.001 moles
(0.001 moles)(176.1g/mol) = 0.1761 grams

Results

Figure (a)
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Figure (b)
Screen Shot 2017-11-26 at 6.19.43 PM.png
Figure (c)
Screen Shot 2017-11-26 at 6.20.11 PM.png
Figure (d)
Screen Shot 2017-11-26 at 6.20.31 PM.png
Figure (e)
Screen Shot 2017-11-26 at 6.20.50 PM.png
Figure (f)
Screen Shot 2017-11-26 at 6.21.00 PM.png

Figures (a) through (f) display the absorbance spectra of the gold nanoparticle mixed with each of the prepared reducing agents solutions. Figure (a) displays the absorbance spectrum of the AuNP-Cellulose nanocrystal solution, with no apparent peaks. Figure (b) displays the absorbance spectrum of the AuNP-Chitosan solution, with no observed peaks. Figure (c) displays the absorbance spectrum of the AuNP-Fructose solution, with an observed peak at wavelength 250nm. Figure (d) displays the absorbance spectrum of the AuNP-Galactose solution, with an observed peak at wavelength 250nm. Figure (e) displays the absorbance spectrum of the AuNP-H2AsCt solution, with a slight observed peak at wavelength 600nm. Figure (f) displays the absorbance spectrum of the AuNP-Na3Ct solution, with an observed steep peak at wavelength 550nm.