User:Sanuja Mohanaraj/Notebook/Experimental Biochemistry 1 Fall 2017/2017/10/10

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Stock Solutions Preparation

4mM AgNO3 Stock Solution
0.0067948 grams of AgNO3 was first transferred into a 10mL volumetric flask, followed by the addition of deionized water to the mark to yield a final concentration of 4mM.

20mM Zinc Acetate Stock Solution
0.036696 grams of zinc acetate was first transferred into a 10mL volumetric flask, followed by the addition of deionized water to the mark to yield a final concentration of 10mM.

35mM Histidine Stock Solution
0.13576 grams of histidine was first transferred into a 25mL volumetric flask, followed by the addition of deionized water to the mark to yield a final concentration of 35mM.

35mM Cysteine Stock Solution
0.106015 grams of cysteine was first transferred into a 25mL volumetric flask, followed by the addition of deionized water to the mark to yield a final concentration of 35mM.

Nanoparticles Preparation

A total of twelve solutions using the gold, silver and zinc stock solutions were prepared according to the table below:
Screen Shot 2017-11-22 at 6.33.10 PM.png

Calculations

4mM AgNO3 Stock Solution
4mM = moles / 10mL
moles = 4e-05 moles
(4e-05 moles)(169.87 g/mol) = 0.0067948 grams

20mM Zinc Acetate Stock Solution
20mM = moles / 10mL
moles = 2e-04 moles
(2e-04 moles)(183.48g/mol) = 0.036696 grams

35mM Histidine Stock Solution
35mM = moles / 25mL
moles = 8.75e-04 moles
(8.75e-04 moes)(155.15g/mol) = 0.13576 grams

35mM Cysteine Stock Solution
35mM = moles / 25mL
moles = 8.75e-04 moles
(8.75e-04 moles)(121.16g/mol)=0.106015 grams

Results

IMG 0875.jpg

The figure above displays the four solutions that were mixed using the 10mM gold stock solution. According to the figure above, Sample 2 was able to form the colloidal gold nanoparticle solution.

IMG 0876.jpg

The figure above displays the four solutions that were mixed using the silver nitrate stock solution. According to the figure above, there is a slight formation of gold nanoparticles in all four samples.

IMGA 0877.jpg

The figure above displays the four solutions that were mixed using the zinc acetate stock solution. According to the figure above, there is no presence of gold nanoparticles in any of the four samples.