User:Samiye Yaman/Notebook/Lab 571/2012/04/04

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Testing the effects of microwave-assisted base hydrolysis in order to determine the concentration of free BSA in solution.


-Add 40 μL of BSA or AuNP solution (synthesized on 02/15/12)to 40 μL 4 M NaOH.

-Add 2μL 450 mM DTT.

-Heat in microwave for 1 min.

-Take UV-vis data.


Absorbance vs wavelength 4-4-12.png