User:Samiye Yaman/Notebook/Lab 571/2012/04/04

From OpenWetWare
< User:Samiye Yaman‎ | Notebook‎ | Lab 571‎ | 2012‎ | 04
Jump to: navigation, search
BDLlogo notext lr.png Biomaterials Design Lab Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png




Objective

Testing the effects of microwave-assisted base hydrolysis in order to determine the concentration of free BSA in solution.

Description

-Add 40 μL of BSA or AuNP solution (synthesized on 02/15/12)to 40 μL 4 M NaOH.

-Add 2μL 450 mM DTT.

-Heat in microwave for 1 min.

-Take UV-vis data.

Data

Absorbance vs wavelength 4-4-12.png