User:Samiye Yaman/Notebook/Lab 571/2012/04/04
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Testing the effects of microwave-assisted base hydrolysis in order to determine the concentration of free BSA in solution.
-Add 40 μL of BSA or AuNP solution (synthesized on 02/15/12)to 40 μL 4 M NaOH.
-Add 2μL 450 mM DTT.
-Heat in microwave for 1 min.
-Take UV-vis data.