User:Samiye Yaman/Notebook/Lab 571/2011/11/16
|Biomaterials Design Lab||Main project page|
Previous entry Next entry
Synthesizing nanoparticles and testing the effects of different solution on the fibers
Part 1 Synthesizing nanoparticles
-1ml of BSA, 0.5 mL of HAuCl and 8.5 ml of water was placed in to a test tube.
-The test tube was placed in to the oven which was at 70degree celcius for 3 hours. (Concentration of BSA is 30uM and HAuCl is 4.17uM.)
-epi tube 2-1 from 15/11/2011 placed in to the heat block again.
-Epi test tube 1 was prepared. 100ul BSA, 50uL HAuCl4, 850 ul water
-Epi test tube 2 was prepared. 25 uL HAuCl4,50uL BSA, 925 uL water. (with this order)
-Epi test tube 3 was prepared. 50uL BSA, 25 uL HAuCl4, 925 uL water.(with this order)
Part 2 Testing the effects of different solution on the fibers. Fibers are added to 4 different solutions.
-Solution 1 had 1 ml of 1M Tris
-Solution 2 had 1 ml of 6M of NaOH
-Solution 3 had 1 ml DMSO.
-Solution 4 had 1 ml DMSO.
The large reaction tube= a dark purple color.
Epitube 1:medium pinkish purple color.
Epitube 2:light pinkish purple color
Epitube 3: light pinkish purple color.
Solution 1 1: fibers did not dissolve.
Solution 2: fibers did not dissolve spontaneously however, fibers dissolved after one week.
Solution 3: fibers dissolved spontaneously. a week later, most of the fibers were dissolved and dark purple solution was left.
This area is for any observations or conclusions that you would like to note.