User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/24

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DNA Validation

Digestion

10 μL DNA
 2 μL 10X NEBuffer 4 -->  52 μL
 1 μL NdeI           -->  26 μL
 7 μL MilliQ         --> 182 μL

37°C for 1 hr.

Expected Patterns

  • Good: 6040, 1416bp
  • Empty: 6645bp

Gel

  • 0.8% Agarose w/ EtBr
  • All colonies gave correct pattern except for 2 and 23