User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/23

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Colony Screen

  • Poured off 1-1.5 mL of each colony into an eppendorf tube.
  • Added 15 μL of 0.1M IPTG to each 4 mL culture.
  • Shook at 37°C for 2.5 hrs.
  • Screened each culture for green fluorescence
    • High background autofluorescence
    • No colonies stood out