User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/21

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Ligation Reaction 1.67 μL pMAL-c2X Digest (50 ng) 1.33 μL PCR Product Digest (37 ng) 7 μL MilliQ 10 μL Quick Ligase Buffer 1 μL Quick Ligase Conditions Incubate at room temperature for 1 min Control 1 sample replaces PCR Product Digest with MilliQ water. Transformation XL1-Blue Competent Cells Plated onto 4 Amp plates (3 sample, 1 control)