User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/16

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Digests Reactions pMAL-c2X 10 μL pMAL-c2X (2 μg) 5 μL 10X NEBuffer 4 .5 μL 100X BSA 1 μL XbaI 1 μL SbfI 32.5 μL MilliQ GFPst PCR Product 25 μL PCR Product 5 μL 10X NEBuffer 4 .5 μL 100X BSA 1 μL XbaI 1 μL SbfI 17.5 μL MilliQ Conditions 37°C for 1.5 hrs. Heat inactivate for 20 min at 65°C.