User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/16

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Digests

Reactions

pMAL-c2X

  10 μL pMAL-c2X (2 μg)
   5 μL 10X NEBuffer 4
  .5 μL 100X BSA
   1 μL XbaI
   1 μL SbfI
32.5 μL MilliQ

GFPst PCR Product

  25 μL PCR Product
   5 μL 10X NEBuffer 4
  .5 μL 100X BSA
   1 μL XbaI
   1 μL SbfI
17.5 μL MilliQ

Conditions

  • 37°C for 1.5 hrs.
  • Heat inactivate for 20 min at 65°C.