User:Robwarden/Notebook/GFP-RGD Force Sensor/2009/03/10

Project name

Main project page Next entry

GFPst PCR Primer Dilutions Stock diluted to 100μM Working concentration diluted to 10μM Reaction 10 μL 5X HF Buffer 1 μL 10mM dNTPs 2.5 μL Forward Primer 2.5 μL Reverse Primer 1 μL GFPuv Plasmid (143.4 ng) 0.5 μL Phusion Hot Start Polymerase 32.5 μL MQ Thermal Cycle Cycles Temp (°C) Time 1 98 0:30 35 98 0:10 68 0:30 72 0:30 1 72 5:00 4 ∞ Validation 0.8% Agarose Gel w/ EtBr Strong band seen & extracted DNA purified with Qiagen kit