User:Robwarden/Notebook/Divalent Ligand Validation/2009/03/14
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Make New Amp Plates
LigationReactions
Setup9.4 μL H1C/H1Y/Water digest 0.6 μL pMSCVN digest 10 μL Quick Ligation Buffer 1 μL Quick Ligase ConditionsIncubate 5 min at room temperature. TransformationReaction50 μL XL10-Blue Supercompetent Cells 0.85 μL beta-mercaptoethanol 5 μL Ligation Product Conditions
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