Objective
Data analysis for reactions run on March 15
Data Workup protocol for all Main UV-VIS Machine analysis and Fluorescence Spectroscopy
UVVis
- import data into excel
- Blank sample data by subtracting water blank absorbance values for before and after reaction
- Absorbance against wavelength
- Monitor maximum absorbance wavelengths before and after
- monitor absorbance wavelengths
Fluorescence
- import data into excel
- subtract water blank sample data from nanoparticle sample data (before and after)
- compute integral for each sample from between 305nm-470nm (peak size and cut off ends to avoid noise in integral calculation)
- calculate ratios of before and after of integrals for same same (just before and after)
- monitor fluorescence peak emission wavelengths
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