Objectives
- UV/Vis and Fluorescence Spectra run for samples after reaction
- Data analysis for UV VIs and Fluorescence data collected
Materials
- Main UV/Vis machine was used to record UV/VIS Spectra of Casein AgNPs, not ocean optics.
- A water blank in the same quartz cuvette was record ed for blanking in data analysis
Procedure
- UV/Vis spectra of reaction mixes were taken from 200-800nm
- Fluorescence spectra was run. Reactions excited at 280nm for tryptophan and tyrosine fluorescence before and after reaction. Scanned emission spectra from 300-540nm ( to avoid excitation peaks at 280 and 570nm)
- UV/Vis and Fluorescence Spectroscopy data can be found at the link SRB Lab Notebook Data
Results
- Color change notes and UV-VIs/ Fluorescence information can be found at the link SRB Lab Notebook Data
- Whey and soy gold were in neutral reaction, pea, casein and rice silver protein were basic - all experience color change (silver=yellow, red/purple = gold)
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