User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/25

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Spermatogenesis

  • Dissected Experimental and control flies that were heat shocked on Thursday.
  • Fix with 4% Formaldehyde PBS Solution
    • 30 minutes
  • Wash 3 x 5 minutes in 1x PBS-T
  • Incubate 30 minutes with PBS 0.6% Triton.
  • Wash once with 1x PBS-T
  • Block in 2% NGS
    • O/N at 4C