User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/18

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Spermatogenesis

  • Heather incubated with primary antibodies O/N. Using anti-bam and anti-vismay on the two samples.
  • Wash 3 x 10 minutes in 1x PBST
  • Incubate with secondary antibody in 2% NGS (1:500, 488 - Bam; anti-mouse, vismay; anti-guinea pig)
    • 2hr at RT or O/N at 4C
  • Wash 3 x 10 minutes in 1x PBST
  • Stain with ToPro3 1:1333 in 2% NGS
    • 25 mins at RT
  • Wash 4 x 5 minutes in PBST
  • Mount in Vectashield