User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/11

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  • Turn experimental, control, FRT, and FLP-Out crosses
  • Experimental and control crosses
    • Stain samples in 1:1000 Topro in 1% NGS for 30'
    • Wash 4x5 mins in PBS-T
    • Finish dissecting and mount
  • HS hsFlp/Y; CD2 Flp Out/W for 2 hours @ 37C. Dissect and fix Friday.