User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/07

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Spermatogenesis

  • Continue Dissections

  • Wash 3 x 15 minutes in 1x PBS-T
  • Incubate with secondary antibody in 2% NGS (1:500, anti-guinea pig, 488nm)
    • 2hr at RT
  • Wash 3 x 15 minutes in 1x PBST
  • Incubate at 4C over weekend. DNA stain/mount on Monday/Tuesday