User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/05/06

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  • Dissecting Control and Experimental heat shocked flies

  • Dissect testes in 1x PBS Solution. Store in eppendorf with 1x PBS until dissections are finished.
  • Fix with 4% Formaldehyde PBS Solution
    • 30 minutes at RT
  • Wash 2 x 5 minutes in 1x PBS-T
  • Incubate 30 minutes with PBS 0.6% Triton.
  • Wash once with 1x PBS-T
  • Block in 2% NGS
    • 1hr at RT
  • Incubate with primary antibody in 2% NGS (anti-Vismay 1:4000)
    • O/N at 4C