User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/02/03

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Spermatogenesis

  • Ran gel of yesterday's PCR
    • 1hr 20 min @ 120V
    • Somewhat inconclusive results, but 5.1 is definitely positive. Will most likely confirm via cross to Flp-out.
  • Observed tdt-Myb, gfp-PCNA
    • Too much background in tdt-Myb channel
  • Dissected tdt-Myb, gfp-PCNA
    • Orange and Peach/Yellow eyes
    • Fix with 4% Formaldehyde PBS Solution
      • 20 minutes
    • Wash 3 x 3 minutes in 1x PBS-T
    • Incubate 25 minutes with PBS 0.6% Triton.
    • Wash once with 1x PBS-T
    • Block O/N 1:1000 in 2% NGS