User:Robert J. Schiemann/Notebook/Lipsick Lab/2010/01/27

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Spermatogenesis

  • PCR for FLPase
    • New negative controls (extracted using the Fly PCR protocol):
      • FM7i
      • w1118
    • Other reactions same
      • Positive control
        • hsFLP122
      • Experimental
        • 1, 5.1, 5.2, 8
    • Run using neo protocol
      • 94C melting
      • 52C annealing
      • 72C extension for 45'