- With gfp-myb, rfp-h2av samples: Heather performed the following yesterday (1/21/10):
- Incubate with primary antibody in 2% NGS
- O/N at 4C
- Anti-sa with 1:100 anti-sa from guinea pigs
- Anti-bam with 1:5 anti-bam from mouse
- Totpro with nothing, continued blocking
- Wash 3 x 10 minutes in 1x PBS-T
- Incubate with secondary antibody in 2% NGS
- (1:500 of respective secondaries conjugated with 633 fluorophore)
- Except totpro, remained in 1x PBS-T
- 2hr at RT
- Wash 3 x 15 minutes in 1x PBS-T
- Keep over the weekend in PBS-T.
- Will stain totpro sample with totpro on Monday and mount the anti-bam and anti-sa samples.