User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/12/03

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Spermatogenesis

  • Change 2% NGS buffer and add 1:20 anti-armadillo
  • Incubate 2 hours 15 minutes @ RT.
  • Wash 3 x 15' at RT
  • Incubate with secondary antibody (Goat anti-mouse 488) @ 4C O/N.