User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/05

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Spermatogenesis

  • anti-PCNA, anti-BAM stained GFP-Myb, RFP-H2av
    • Wash 3 x 15' @ RT in PBS-T

To D:

  • tdt-Myb
    • Stain 30' @ RT with oliGreen (1:500) and RNAse A(1:10) in 2% NGS
    • Wash 4 x 5' in PBS-T
  • 9 Day
    • Stain 30' @ RT with Topro3 (1:1000) in 2% NGS
    • Wash 4 x 5' in PBS-T
  • All
    • Mount in Vectashield