User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/04

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Spermatogenesis

  • 9 Day Heat Shock
    • Dissect Control and Experimental
    • Fix 20' in 4% formaldehyde @ RT
    • Wash 4 x 3' in PBS-T @ RT
  • GFP-Myb, RFP H2Av
    • Wash 3 x 15' in PBST
    • Stain 2 hrs @ RT with secondary
      • anti-PCNA mouse (use anti-mouse 633)
      • anti-BAM mouse (use anti-mouse 633)