User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/11/03

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Spermatogenesis

  • Dissect GFP-Myb for staining with the other Anti-BAM(1:5) Anti-PCNA(1:500).
    • Poor testis for some reason (old flies?)
    • 14 dissected total in two groups of 7
  • Dissect tdt-Myb for staining (30' at RT) with Oligreen(1:500) (treat with RNAse(1:10) during incubation)
    • Decent testis
    • 9 flies dissected
  • Fixed all testis in 4% Formaldehyde in PBST for 20'
  • Washed 3 x 5' in 1x PBST
  • Block O/N in 2% NGS