User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/16

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Spermatogenesis

  • [ubi-RFP]
    • Stain 30' in Totpro 1:500
    • Wash 3x10' or 4x5' in PBS-T
    • Mount immediately.
  • [GFP-myb, RFP-H2Au]
    • Incubate w/ 1:5 dilution of a-Cyclin B (Mouse Antibody) O/N.
  • Crosses
    • Collected two virgin [MH107,hsFLP/FM7Ag ; mRFP, myb+, FRT42D/CyoAg]
    • Will start cross tomorrow