User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/15

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Spermatogenesis

  • Dissections of 11 [ubi-RFP] and 13 [GFP-myb, RFP-H2Au] lines into PBS.
  • Fix 30' in 4% Formaldehyde, PBS-T at RT.
    • Start 5:55
    • End 6:25
  • Wash 4x5'
  • Block in 2% NGS 0/N