User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/02/03

Drosophila melanogaster Genetics

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Spermatogenesis Turned over crosses Collected two [MH107,hsFLP/Y ; mRFP, myb+, FRT42D/FRT42D] males. Heat shocked at 37C for 1 hour 11:50 AM - 12:50PM Will dissect on Thurs, Feb 5. with the following stains: Either Spectrin w/ 488 Secondary or Falloidin 488 Totpro for DNA Will collect more males on Thurs, Feb 5. and heat shock. Will dissect on Tues, Feb 10. FRT/RFP Recombination Collected virgins of the three crosses and set up the following cross: V[mRFP/FRT] x [Sc/Cyo] Will collect non-scutoid males with orange-red eyes and set up individual crosses to V[Sc/Cyo]. Will collect more virgins Feb 4 and Feb 5. Turned over crosses