User:Rebeca Rodriguez/Notebook/Chem 471/2015/10/13
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5 1 mL AuNP fiber samples were centrifuged (and supernatant fluid removed), and combined into one 1 mL sample in Tris/NaCl buffer solution. A small volume of proteinase K was added such that the the volume of proteinase K in the cuvette was 1 uM. The degradation of the AuNP fibers was then monitored using the Ocean Optics UV-Vis. Spectra were taken every 2 minutes, with one spectrum taken before the addition of the proteinase K.
Set up protocols for the Ocean Optics UV-Vis can be found here.
This is the spectra for the first 60 minutes of the degradation of AuNP fibers by proteinase K.
This figure displays the peak absorbance values from the first spectra absorbance plotted time.