User:Rebeca Rodriguez/Notebook/Chem 471/2015/10/13

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Objective

  1. Run Ocean Optics Kinetics on AuNP Fibers by 1 uM proteinase K.
  2. Data analysis!

Protocol

5 1 mL AuNP fiber samples were centrifuged (and supernatant fluid removed), and combined into one 1 mL sample in Tris/NaCl buffer solution. A small volume of proteinase K was added such that the the volume of proteinase K in the cuvette was 1 uM. The degradation of the AuNP fibers was then monitored using the Ocean Optics UV-Vis. Spectra were taken every 2 minutes, with one spectrum taken before the addition of the proteinase K.

Set up protocols for the Ocean Optics UV-Vis can be found here.

Data

Ocean Optics 10-13.png

This is the spectra for the first 60 minutes of the degradation of AuNP fibers by proteinase K.

Ocean Optics Analysis 10-13.png

This figure displays the peak absorbance values from the first spectra absorbance plotted time.