Two groups will be working on determining fluorophore concentration. (Alicia/Madeleine and Becky/Eleni/Melvin) The other two groups will be working on diffusion. (Jake/James/Mary and Andrew/Michael/Tami).
NOTE: The groups working on fluorophores will need to space to the groups working on diffusion as their research is very time sensitive.
- Make stock concentrations (both groups can use the same solutions)
- Take UV-Vis and Fluorescence spectra of these samples.
- Make a calibration curve based on UV-Vis.
- Compare your data to some published values
- Make a calibration curve based on the fluorescence.
- In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.
- One group will use a PVA film
- The other group will use a PVA/clay film
- Set the film between the two chambers
- Add liquid to each chamber
- One chamber gets water
- The other chamber gets 20ppm Malachite green
- Withdraw 200uL from each chamber at the 5 minute mark and place in their own eppy tube
- NOTE: I want the groups to coordinate so that their 5 minutes don't overlap with one another.
- NOTE: You'll want to use a small volume cuvette
- NOTE: You'll also want a 0minute measurement for each.
- NOTE: You'll likely have to dilute the sample from your chamber with the 20ppm. So, when you return the sample, only return the non-diluted sample.
- Measure the UV-Vis spectrum for both samples. Return each to the chamber it originally came from
- Take measurements at the following times
- 15 minutes
- 30 minutes
- 1 hour
- 2 hours
- right before you leave lab
- It would be great if someone could stop by on Thursday to take a measurement then.
- You'll be taking measurements on Friday too.
- During each of this, it would be best to start working up the data right away.
0.0120g of R6G in 10mL needed to make a 2500uM stock solution. This solution was used to make 2.0uM. The solution was diluted further (1:5), to create a 500uM stock solution in order to make the dilutions for 0.1, 0.5, 1.0, and 1.5.
|Stock Concentrations (uM)
||Amount of Stock Solution Needed (mL)
| Exitation Wavelength
| Slit Width
| Scan Speed
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