User:Raf Aerts/Notebook/Open Coffee/2009/02/24

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Coffea arabica DNAx :: lysis buffers C0/C1

  • Nanodrop ND-1000 Spectrophotometer setup
    • nucleic acid DNA-50
    • blank: 1.5 μL CE buffer
    • samples 1.5 μL
  • Results
    • C0 buffer gives high peak near 230 in visual reference graph
    • C1 buffer shows no peak at all
    • Both C0 and C1 yield low concentration, low purity DNA (all 260/280 and 260/230 < 1.8)
      • C0: 32.7 ng/ul
      • C1: 8.5 ng/ul

Ndcac0c1.jpg