User:Rachel Gill/Notebook/Chem 681 2018/2019/03/27

From OpenWetWare
Jump to navigationJump to search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      
BDLlogo notext lr.png Biomaterials Design Lab Report.pngMain project page
Resultset previous.pngPrevious entry      


Objective

Crosslink chitosan that has been neutralized with 0.005 M glutaraldehyde.

Lab Protocol

Used previous pump set up to crosslink chitosan using mineral oil instead of olive oil and 1 mL/min for carrier fluid flow rate on grey pump.

Data

  • the neutralized chitosan did not crosslink with the diluted gultaraldehyde. tried again with a more concentrated glutaraldehyde mixture like last semester (1/2 mL of glutaraldehyde with 2.5 mL of water in 3 mL syringe) also did not work.
  • made more dissolved chitosan in 5% HC2H3O2 to try to crosslink with the full 8% gultaraldehyde next time.
  • retired to make MG clay solutions for UV-Vis analysis
clay (g) start MG (ppm) MG (g)
0.1028 25.0 5.0313
0.1003 49.977 5.0098
0.1072 74.9 5.0385
0.1001 98.7 5.0115
0.1032 247.0 5.0300

Notes