User:Rachel Gill/Notebook/Chem 681 2018/2019/01/25

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Objective

Create more PVA/clay beads for ICP analysis of Pb and Hg stock solutions. Create malachite green stock solution for Na-montmorillonite (aka closite) for UV-Vis analysis.

Lab Protocol

MW of MG = 364.1 g/mol

Following bead procedure from previous semester with adjustment in carrier fluid rate (1.0 mL/min). This change has occurred due to stalling of grey pump at the faster rate, 2.0 mL/min. 1.0 mL/min seems to have no affect on aggregation of the beads and does not cause the pump to stall when running for longer periods of time. An added benefit to this new rate, the carrier fluid, and the PVA/clay and gluteraldehyde syringes run out at the same times. This will make refilling syringes easier when making multiple batches of new beads in the future.

Data

Malachite Green Stock

0.000250/1 = x/50

x = 0.0125 g MG needed

actual used: 0.01237 g actual total solution: 50.0395 g actual concentration 247 ppm

Malachite Green diluted samples

  1. to make 100 ppm: 20.0440 g of 247 ppm used in 50.1717 g total solution. actual concentration 98.7 ppm.
  2. to make 75 ppm: 38.0060 g of 98.7 ppm used in 50.0531 g total solution. actual concentration 74.9 ppm.
  3. to make 50 ppm: 33.3775 g of 74.9 ppm used in 50.0225 g total solution. actual concentration 49.977 ppm.
  4. to make 25 ppm: 25.0300 g of 49.977 ppm used in 50.0249 g total solution. actual concentration 25.0 ppm.

Notes