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Objective
- Make 10% Chitosan with 10% HCl to see if that can be used in place of PVA to hold montmorillonite. Learn how to used the SEM and take photos of PVA and PVA/clay beads.
Lab Protocol
- Chitosan solution:
- Tube 1: 5.03225 g chitosan, 45.01566 g 10% HCl
- Tube 2: 4.96347 g chitosan, 45.19017 g 10% HCl
SEM How to
- Turn key to start
- Wait for Evac to stop blinking then click vent
- Open chamber
- Prepare samples by cutting carbon fiber tape and putting then on sample stand that has small circles on it.
- Slide sample stand on gold sample holder curve part faces back
- Evac, raise beam 10 mm (knob on top of sample chamber)
- turn on computer
- chamberscope - camber for inside
- in touch scope (without circle) open once evac stops blinking
- observe turns on beam, clicking on screen moves camera to that location
- WB on bottom left, set to 10 mm
- to pull up stage selecting click grey arrow in right most corner
- ACB when you first look at sample (auto contrast and brightness)
- change probe current, zoomed in low 30, zoomed out high 50
- zoom in as much as you want, click RDC to pull up small window to play with course and fine adjustment (bottom right, use mouse wheel to adjust)
- to save image: settings -> SEM -> user name image -> change count number to 1 for first sample
- click on camera icon to take picture
- click fast when done
- observe turn off beam, vent, and raise beam 20-30 mm
- when vent stops blinking take out sample, close chamber and press evac
- when evac stops blinking shut down software then turn key to 0 (off)
Data
PVA beads
PVA/clay beads
New Samples of PVA beads DSC
- sample 1: pan mass 40.44 mg, sample mass 3.83 mg
- sample 2: pan mass 40.53 mg, sample mass 3.73 mg
- reference pan: 40.12 mg
MG dye uptake for PVA beads
- sample 1: beads 0.10146 g, 0.5 ppm MG 5.00681 g
- sample 2: beads 0.10023 g, 1.00 ppm MG 5.00132 g
- sample 3: beads 0.10032 g, 1.53 ppm MG 5.04375 g
- sample 4: beads 0.10070 g, 3 ppm MG 5.02209 g
- sample 5: beads 0.10068 g, 8.88 ppm MG 5.02565 g
- sample 6: beads 0.10010 g, 15.3 ppm MG 5.01107 g
- sample 7: beads 0.10009 g, 25.3 ppm MG 5.00905 g
- sample 1A: beads 0.10007 g, 0.5 ppm MG 5.02098 g
- sample 2A: beads 0.10089 g, 1.00 ppm MG 5.02039 g
- sample 3A: beads 0.10088 g, 1.53 ppm MG 5.01322 g
- sample 4A: beads 0.99983 g, 3 ppm MG 5.00572 g
- sample 5A: beads 0.10095 g, 8.88 ppm MG 5.02091 g
- sample 6A: beads 0.10050 g, 15.3 ppm MG 5.00876 g
- sample 7A: beads 0.10083 g, 25.3 ppm MG 5.02333 g
Notes
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Project name
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