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Objective
Analyze last weeks sample with SECM and create new beads using montmorillonite in PVA.
Lab Protocol
- Same set up as designed on last week.
- May need to use oil as carrier fluid today because ethyl acetate is most likely cause of clumping up of cross-linked particles.
Data
- rate of olive oil changed to 2 mL/min
- collected more beads and lyophilized them so they can be imaged
- grey pump, carrier fluid, olive oil. Two 60 mL syringes with rate of 2 mL/min.
- red pump, PVA and gluteraldehyde, rate 0.05 mL/min.
- PVA and gluteraldehyde are mixed in y-junction with the shortest tube possible to connect the y to the cross junction where the olive oil will pinch off the composite mixture into beads.
- gluteraldehyde filled in 3 mL syringe with 1/2 mL of 8% gluteraldehyde and then 2 1/2 mL filled with distilled water.
- 10% PVA (5.8 g PVA [Mw 31-50,000 kDa] in 5.8 g Di H2O) in 10% HCl
Notes
- tubing needs to be taught to prevent clumping of beads inside tubing and to help optimize flow.
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