User:Rachel Gill/Notebook/Chem 681 2018/2018/10/24

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Objective

Analyze last weeks sample with SECM and create new beads using montmorillonite in PVA.

Lab Protocol

  • Same set up as designed on last week.
  • May need to use oil as carrier fluid today because ethyl acetate is most likely cause of clumping up of cross-linked particles.

Data

  • rate of olive oil changed to 2 mL/min
  • collected more beads and lyophilized them so they can be imaged

20181024 112426.jpg

  • grey pump, carrier fluid, olive oil. Two 60 mL syringes with rate of 2 mL/min.
  • red pump, PVA and gluteraldehyde, rate 0.05 mL/min.
    • PVA and gluteraldehyde are mixed in y-junction with the shortest tube possible to connect the y to the cross junction where the olive oil will pinch off the composite mixture into beads.
    • gluteraldehyde filled in 3 mL syringe with 1/2 mL of 8% gluteraldehyde and then 2 1/2 mL filled with distilled water.
    • 10% PVA (5.8 g PVA [Mw 31-50,000 kDa] in 5.8 g Di H2O) in 10% HCl

Notes

  • tubing needs to be taught to prevent clumping of beads inside tubing and to help optimize flow.